Hoefer HE33 Manual do Utilizador descarregar pdf (Página 22)

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Sample loading buffer

Loading buffer

(5X, 25% Ficoll 400, 0.25% Bromophenol blue

†

,10 ml)

Deionized H

O to 7.0 ml

Ficoll 400 2.5 g

Bromophenol blue (FW 691.9) 25.0 mg

Deionized H

O to 10.0 ml

Add to sample in proportion so that 1/5 of the ﬁnal

volume is loading buffer. (Loading buffer increases

solution density.)

Note 1: Sucrose or glycerol may be used instead of

Ficoll 400.

Note 2: Xylene cyanol (0.25%), which migrates more

slowly than bromophenol blue, can be added

as an additional marker if desired. The agarose

concentration determines the position of the

dye bands relative to a polynucleotide.

†

Tracking dyes may be omitted to eliminate obscuring and dragging

effects caused by comigration with smaller nucleic acids.

Table 3: Comb speciﬁcations and well volumes

well sample vol.

comb no. of thickness width per 1 mm

code no. wells (mm) (mm) depth (µl)

HE31A-P-1.0 1 prep/2 ref 1.0 44/6 44/6*

HE31A-P-1.5 1 prep/2 ref 1.5 44/6 66/9*

HE31A-8-1.0 8 1.0 6.5 6.5

HE31A-8-1.5 8 1.5 6.5 9.7

HE31A-12-1.0 12 1.0 3.9 3.9

HE31A-12-1.5 12 1.5 3.9 5.8

HE31A-16-1.0 16 1.0 2.6 2.6

HE31A-16-1.5 16 1.5 2.6 3.9

* The preparative combs form two reference wells (for MW

standards), one on each side of the preparative well. The

ﬁrst number is sample volume/mm in the preparative well;

the second is volume/mm in the reference well.

1 2 ... 17 18 19 20 21 22 23 24 25 26

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