Hoefer TE70X Manual do Utilizador

user manualum    TE70X-IM/Rev.C0/07-12TE70X Semi-dry BlottersTE70X Semi-dry transfer unitTE77X Semi-dry transfer unitTE70XP Semi-dry transfer unitTE7

• p2UnpackingUnwrap all packages carefully and compare contents with the packing list, making sure all items arrived. If any part is missing, contact

Specifications TE70X TE77X TE70XP TE77XPElectrode size (cm) 14 × 16 21 × 26 14 × 16 21 × 26Inputs: Voltage (V) 30 (DC)* 30 (DC)* 100

• p42. Operating instructions To transfer proteins, prepare the unit, assemble the stack, and connect to a power supply if necessary. Then run the tra

• p54Prepare the blotting paperFor each gel, cut at least 6 pieces of blotting paper the same size as the gel or slightly smaller. Gauge the thickness

• p66Complete the stacka. Place the pre-wet membrane onto the stack of blotting paper.b. Place the gel on the membrane. Note: Proteins bind to the

• p7Multiple gels: Either lay gels of the same thickness side-by-side (Fig 3), or stack 2 sandwiches layered as shown (Fig 4).For best results, the tr

• p8Blotting paper, (2–3 sheets)Blotting paper, (1 sheet)CellophaneMask (optional)GelMembraneBlotting paper, (2– 3 sheets)GelMembraneFig 4. Transfer s

• p9ElectrotransferTE70X and  TE77X1The power supply should be switched off and both the current and voltage controls set at zeroThen plug the color-c

• p10TE70XP and  TE77XP1Turn the instrument on using the POWER button on the front keypad. The instrument works by setting the current and time, and t

• p11power ON/OFFDuring the  transferTE70XP  and the TE77XPA red LED will appear in the lower right corner of the Display when voltage is applied to

• piContents Important Information ... iiWaste Electrical and Electronic Equipment (WEEE) ...

• p12After the  transfer is completeTE70X  and TE77XTurn off the power supply and disconnect the leads from the power supply.TE70XP  and TE77XPAfter a

• p133. Care and maintenance• Do not autoclave or wash the unit in a dishwasher.• Do not immerse the unit in water!Rinse the cathode in the cover an

• p144. Troubleshootingproblem  solution Incomplete transfer Blank or faint areas on Remove trapped air pockets between the gel andthe membrane

• p15problem  solution Open circuit or no output current  Push circuit protection reset button located next tofor TE70X or TE77X   the input leads. To

• p16problem  solution Inefficient binding to membrane Chemical parameters Prepare transfer buffer without SDS. (SDS can improve transfer efficiency

• p175. Electrotransfer notes• Run the transfer as soon as possible after electrophoresis to minimize protein diffusion within the gel.• Stacked g

• p18CAPS  buffer, 1X(10 mM CAPS, pH 11.0, 1 liter)CAPS (FW 221.3) 10 mM 2.2 g [3-(cyclohexylamino)-1-propanesulfonic acid]Dissolve in 600 ml dist

• p196. BibliographyBjerrum, O.J., Larsen, K., and Heegaard, N., CRC Handbook of Immunoblotting of Proteins 1, Section 7. CRC Press (1988).Gallagher,

• p207. Customer service informationTechnical service and repairHoefer, Inc. offers complete technical support for all of our products. If you have an

• p218. Ordering informationproduct   qty.  code no.TE70X Semi-dry Transfer Unit, 14 × 16 cm  1 TE70X Includes 25 sheets of blotting paper, 50 sh

• piiImportant Information  – English• If this equipment is used in a manner not specied by Hoefer, Inc. the protection provided by the equipment ma

• p22Related productsproduct     code no.SE600 Chroma SE600X-15-1.5MiniVE Vertical Electrophoresis system SE300-10A-1.0PBS300B Powe

• p23Hoefer, Inc. 84 October Hill Road Holliston, MA 01746Toll Free: 1-800-227-4750 Phone: 1-508-893-8999 Fax: 1-508-893-0176 E-mail: support@hoeferin

• piii80%). Undgå kondensation ved lade enheden equili-brate til omgivende temperatur ved tageen instru-mentets fra et koldere til et varmere miljø. •

• pivil devrait être seulement utilisé par les opérateurs convenablement entraînés. Lire ce manuel entier avant utiliser l’instrument et l’usage seule

• pvsopra di 80%). Evitare la condensazione lasciando l’unità equilibra alla temperatura ambiente quando portare lo strumento da un più freddo a un am

• pvi• Este instrumento é projectado de acordo com o EN61010-1:2001 condição de segurança eléctrica. Não obstante, deve ser usado só por operadores a

• pviiEnglishFrenchGermanItalianSpanishSwedishThis symbol indicates that the waste of electrical and electronic equipment must not be disposed as unso

1. TE70X Semi-dry Blotters:  DescriptionThe TE70X, TE77X, TE70XP, and the TE77XP semi-dry blotters rapidly transfer proteins from polyacrylamide gels