Hoefer Single Channel Variable Pipette Manual do Utilizador descarregar pdf (Página 7)

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7. MAINTENANCE

Use 70% ethanol and a soft cloth or lint free tissue to wipe the external pipette surfaces

clean.

To clean in the interior of the pipette:

1. Hold down the tip ejector.

2. Place the pronged end of the wrench between the tip ejector and the tip ejector

collar to release the locking mechanism.

3. Carefully release the tip ejector and remove the collar.

4. Using the wrench, turn the tip cone counter clockwise to remove the cone.

5. Wipe the piston, O-ring and the tip cone with 70% ethanol. Note: Pipettes with

volumes of 10 µl or less have a fixed O-ring and cannot be accessed.

6. Grease the piston with a light coating of silicone.

7. Reassemble the pipette and then depress the thumb button several times to

lubricate the piston to make sure it does not bind.

8. Check calibration.

8. TROUBLESHOOTING GUIDE

Problem Possible Cause Solution

Tip incompatibility Try a different brand of tip

Liquid left inside the tip

Plastic tip not uniform Attach a new tip

Tip incorrectly attached Reattach tip

Tip cone fouled Clean tip cone

Pipette has material inside

cone

Clean and grease O-ring

and piston; clean tip cone

O-ring not positioned

correctly or damaged

Replace O-ring

Leakage or pipette volume

too small

Calibration incorrect Recalibrate pipette

Piston damage Clean and grease O-ring

and piston; clean tip cone

Solvent contamination Clean and grease O-ring

and piston; clean tip cone

Push button jammed or

off center

Calibration incorrect Recalibrate pipette

Pipette not aspirating

Material blocking cone tip Clean and grease O-ring

and piston; clean tip cone

Tip ejector jammed

Ejector collar has debris Clean tip cone and ejector

collar

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Hoefer Single Channel Variable Pipette Manual do Utilizador Página 7

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